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Market => Product requests => Topic started by: drkim on May 05, 2013, 12:43 pm

Title: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 05, 2013, 12:43 pm
Is there any source of either Ergot culture, spores, or other ergot derivatives on SR?

I know the Ron Paul guy i supposed to have a source of ergot, to get your own culture, but since i havnt seen any info on the forums for users who have succesfully got themself some kind of ergot, i am sceptical. 

The best thing would for sure to get some spores, and make an ergot culitvation.

Doesnt anyone have a source? The world needs more ergot, and thus more clandestine labs :D

Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 06, 2013, 03:46 am
sorry a hard find
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: PerPETualMOtion on May 06, 2013, 05:24 am
Hmmm.... is that the purple fungus that attacks wheat crops? The stuff that poisoned the Greeks or other Tribe of Old.

Yes... are there any vendors that live near wheat fields? Just take a clean mason jar and seal that shit up along with some fresh wheat.

Ergot begets LSD and other happy synthees.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 06, 2013, 08:17 am
Thats what im about to do soon, since i dont find any source, i will just have to go hunting rye, and hope to stumble upon the ergot :) Once found all is good, then i can be the source :D
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: C20H25N3O on May 06, 2013, 02:33 pm
I am pretty sure that I've seen in the past between RP sells[I mean in the feedback section] an ergot culture but you  need to buy his LSD guide to have access to this source
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 06, 2013, 04:06 pm
theirs a route via ergovamine. quite rare, not sure if you v heard of it, wont even come on Google but it does exist and so does the route
btw there is a spelling error on ergovamine, ill reference it later and correct the spelling. i thought google would have showed it up, weird
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: ToadStool on May 06, 2013, 04:35 pm
Yes, a source for sterile cultures would be very nice. I have studied ergot for years and did run across a few things someone may find interesting. First of all, the ergot seen growing wild in rye fields or wherever is Claviceps pupurea. Correct me if I'm wrong, but I do think the strain which is cultured by pharmaceutical companies is Claveceps paspali. C. paspali lends itself better for culturing and produces more of the desired alkaloids. You really need C. paspali for culturing purposes. Does anyone know where a slant of C. paspali may be found? Another cool thing I discovered about 30 years ago is the fact that ergot also grows rampantly on the salt water marsh grass Spartina (Cordgrass). You can find a ton of this stuff in late fall if you live anywhere near the gulf coast. There is a marina down in Ocean Springs, Mississippi where patches of Spartina grass can be found growing and it is always completed infected with ergot sclerotium every fall. Unfortunately, local laws protect the grass so you must be stealthy if you find some and wish to harvest the ergot sclerotia. Ironically, the grass regenerates itself from rhizomes in the ground rather than from seeds. Since the seed heads are completely covered with ergot, there is no way the seeds could actually survive and germinate. But, the law still says you can't fuck with the grass and you can be sure someone will call the cops if they see you picking the grass. I'm not sure which species of ergot it is that grows on the marsh grass but it does produce a shitload of sclerotia and it does contain active alkaloids. As is true when fooling around with any ergot, wear gloves and be really careful not to poison yourself. Ergotism is extremely nasty and can cause your extremities to develop gangrene due to vascular constriction. Again, exercise caution when handling ergot!! It can make you very sick if not kill you if you ingest enough of it. I'm sure some idiot has probably tried to make ergot tea or something trying to get high only to kill him/her self. Oh well, Darwin's theory holds true.... Enough schooling. If anyone knows where a culture may be found, please let us know. Thanks.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 06, 2013, 04:48 pm
yes there is a country that has masses of ergot but still controlled.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: ToadStool on May 06, 2013, 05:01 pm
I'd love to find a source for good ole ergotamine tartrate. You'd think there would be a source somewhere on the onion. I've often wondered how lab operators obtain the precursors for LSD synthesis. I actually have about 300 mls of diethylamine but it's a zillion years old. I don't know how long it stays good so I have no idea if mine is still useable. I'm sure it's absorbed moisture over the years since the bottle has been opened a few times. Maybe it can be distilled back to an anhydrous state. I just don't know. Anyone know anything regarding shelf life of diethylamine? Any help will be appreciated.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Sensei on May 06, 2013, 05:10 pm
I was just wondering wouldn't it be easier to invest in a vacant land with no neighbors or other grain growers within the area and grow your own Rye and just allow it to become infected with ergot and then collect it? Or what about starting a small patch in some remote area and allowing it to become infected and then collecting and starting a culture from that or is that impractical? I am more curious than informed on this subject, just throwing some thoughts out there.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: PerPETualMOtion on May 06, 2013, 05:11 pm
Yes, a source for sterile cultures would be very nice. I have studied ergot for years and did run across a few things someone may find interesting. First of all, the ergot seen growing wild in rye fields or wherever is Claviceps pupurea. Correct me if I'm wrong, but I do think the strain which is cultured by pharmaceutical companies is Claveceps paspali. C. paspali lends itself better for culturing and produces more of the desired alkaloids. You really need C. paspali for culturing purposes. Does anyone know where a slant of C. paspali may be found? Another cool thing I discovered about 30 years ago is the fact that ergot also grows rampantly on the salt water marsh grass Spartina (Cordgrass). You can find a ton of this stuff in late fall if you live anywhere near the gulf coast. There is a marina down in Ocean Springs, Mississippi where patches of Spartina grass can be found growing and it is always completed infected with ergot sclerotium every fall. Unfortunately, local laws protect the grass so you must be stealthy if you find some and wish to harvest the ergot sclerotia. Ironically, the grass regenerates itself from rhizomes in the ground rather than from seeds. Since the seed heads are completely covered with ergot, there is no way the seeds could actually survive and germinate. But, the law still says you can't fuck with the grass and you can be sure someone will call the cops if they see you picking the grass. I'm not sure which species of ergot it is that grows on the marsh grass but it does produce a shitload of sclerotia and it does contain active alkaloids. As is true when fooling around with any ergot, wear gloves and be really careful not to poison yourself. Ergotism is extremely nasty and can cause your extremities to develop gangrene due to vascular constriction. Again, exercise caution when handling ergot!! It can make you very sick if not kill you if you ingest enough of it. I'm sure some idiot has probably tried to make ergot tea or something trying to get high only to kill him/her self. Oh well, Darwin's theory holds true.... Enough schooling. If anyone knows where a culture may be found, please let us know. Thanks.

Hmmm... Species harvesting.

Here's a wild idea. Watch the weather. When a front is going to carry Midwest  air over your area, put out a ton of sterile, saturated wheat grains or flour--or simply agar trays--until the front passes. Allow inoculation until desired species is obtained. This will only work for avid microbiologists, and of course, industrial-sized grow operations.  ;)

You can get almost any species of mold/bacteria you want if you're patient. Also, watch the UV-index for the traveling front: too much UV kills all the precious spores.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: ToadStool on May 06, 2013, 05:40 pm
I was just wondering wouldn't it be easier to invest in a vacant land with no neighbors or other grain growers within the area and grow your own Rye and just allow it to become infected with ergot and then collect it? Or what about starting a small patch in some remote area and allowing it to become infected and then collecting and starting a culture from that or is that impractical? I am more curious than informed on this subject, just throwing some thoughts out there.

Actually, I think they already do this in Spain or somewhere and grow ergot commercially. I've seen photos of people spraying rye fields with an ergot inoculant to infect the rye. It then does it's thing and grows all over the seed heads producing sclerotium which are then gathered for extraction. I don't know if it's still grown this way today or if it's all produced from lab cultures now. Don't you know it must piss off the farmers who are trying to grow clean grain?? I bet it spreads like wildfire.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 06, 2013, 06:34 pm
Me no chemist but to whoever doing a research on this field i think this will surely help


A)   Get a source for Claviceps Paspali

B)

L-tryptophane and succinamide have great effect on fermentation.

The best composition of culture medium is 7% sorbitol, 3% succinamide, 0.1% L-tryptophane, 0.05 %MgSO4•7H2O, 0.03% KH2PO4 and pH 5.2 adjusted with ammonia. Through the cultivation of 14 days at 24-26℃ in shake flasks, the biggest content of the ergot alkali reaches 95.6 mg/L

C) Large-scale production

Obtain several ordinary 1 gallon jugs.
Place a two-hole stopper in the necks of the jugs.
Fit a short (6 inch) tube in one hole, leaving two inches above the stopper. Fit a short rubber tube to this. Fill a small (500 ml) Erlenmeyer flask with a dilute solution of sodium hypochlorite (NaClO). Extend a glass tube from the rubber so the end is immersed in the hypochlorite.
Fit a long glass tube in the other stopper hole. It must reach near the bottom of the jug and have about two inches showing above the stopper. Attach a rubber tube to the glass tube and fit a short glass tube to the end of the rubber tube.

Fill a large glass tube (1" x 6") with sterile cotton and fit one-hole stoppers in the ends. Fit the small glass tube in the end of the rubber tube into one stopper of the large tube. Fit another small glass tube into the other stopper. A rubber tube is connected to this and attached to small air pump (obtained from a tropical fish store).
With this aeration equipment you can assure a supply of clean air to the Claviceps Paspali fungus while maintaining a sterile environment inside the solution.
Dismantle the aerators. Place all the glass tubes, rubber tubes, stoppers and cotton in a paper bag, seal tightly with wire staples and sterilize in an autoclave.
Fill the 1-gallon jugs 2/3 to 3/4 full with the culture medium and autoclave.
While these things are being sterilized, homogenize in a blender the culture already obtained and use it to inoculate the material in the gallon jugs. The blender must be sterile.

EVERYTHING must be sterile.

Assemble the aerators. Start the pumps. A slow bubbling in each jug will provide enough oxygen to the cultures. A single pump may be connected to several filters.
Let everything sit at room temperature (25 C) in a dark place (never expose ergot alkaloids to bright light - they will decompose) for a period of ten days.
After ten days, adjust the culture to 1% ethanol using 95% ethanol under sterile conditions. Maintain growth for another two weeks.

D) Extract ergot alkaloids

After a total of 22 days growth period, the culture should be considered mature. Make the culture acidic with tartaric acid and homogenize in a blender for one hour.
Adjust to pH 9 with ammonium hydroxide and extract with benzene or chloroform/iso-butanol mixture.
Extract again with alcoholic tartaric acid and evaporate in a vacuum to dryness.
The dry material is the salt (the tartaric acid salt, the tartrate) of the ergot alkaloids, and is stored in this form because the free basic material is too unstable and decomposes readily in the presence of light, heat, moisture, and air.
To recover the free base for extraction of the amide or synthesis to LSD, make the tartrate basic with ammonia to pH 9, extract with chloroform, and evaporate in vacuo.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: uhrwerk on May 06, 2013, 07:09 pm
I would love to see a culture or ergotamine as well, but lets face it; who would go through the trouble of obtaining some ergotamine to only sell it if you can get the profit from a simple reaction and workup ?

Has anybody purchased the LSD manufacturing guide and can say something about it // maybe only confirm if a culture is available would be nice.

Ergotamine and most other ergolines have traditionally been produced by rye infestion because claviceps purpurea submerged culture was neither understood nor stable and high-producing strains were not known at the time hofmann and Stoll made their research. It is very temperate to ferment, so submerged cultures have not yet replaced rye infestion fully. Countries such as Czech, Suiss and Hungary are the biggest producer of ergolines in the world, even the chinese get their products out of the EU.
(this is from memory, out of  "ergot: the genus claviceps")

from: Purification of Laboratory chemicals, 6th ed. (2009);
Diethylamine [109-89-7] M 73.1, b 55.5°, d20/4  0.707, n 1.38637,  pK15 11.38 .   
Dry diethylamine with LiAlH4 or KOH pellets. Reflux with, and distil it from, BaO or KOH.  Convert it  to  the  p-toluenesulfonamide and crystallise to constant melting point from dry pet ether (b 90-120°),  then hydrolyse with HCl, excess NaOH is added, and the amine is passed through a column of activated alumina. Redistil the amine and dry it with activated alumina before use [Swift J Am Chem Soc 64115 1942]. [Beilstein 4III 313.]
§ A polystyrene diethylaminomethyl supported version is commercially available.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 06, 2013, 07:27 pm
I would love to see a culture or ergotamine as well, but lets face it; who would go through the trouble of obtaining some ergotamine to only sell it if you can get the profit from a simple reaction and workup ?


i still heard underground a gram of ET goes for around usd700 and the simple industrial setup can yield half a key every twenty days mate, and lot of advantages are there for choosing paspali instead of purpurea
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 06, 2013, 10:04 pm
I would love to see a culture or ergotamine as well, but lets face it; who would go through the trouble of obtaining some ergotamine to only sell it if you can get the profit from a simple reaction and workup ?

Has anybody purchased the LSD manufacturing guide and can say something about it // maybe only confirm if a culture is available would be nice.

Ergotamine and most other ergolines have traditionally been produced by rye infestion because claviceps purpurea submerged culture was neither understood nor stable and high-producing strains were not known at the time hofmann and Stoll made their research. It is very temperate to ferment, so submerged cultures have not yet replaced rye infestion fully. Countries such as Czech, Suiss and Hungary are the biggest producer of ergolines in the world, even the chinese get their products out of the EU.
(this is from memory, out of  "ergot: the genus claviceps")

from: Purification of Laboratory chemicals, 6th ed. (2009);
Diethylamine [109-89-7] M 73.1, b 55.5°, d20/4  0.707, n 1.38637,  pK15 11.38 .   
Dry diethylamine with LiAlH4 or KOH pellets. Reflux with, and distil it from, BaO or KOH.  Convert it  to  the  p-toluenesulfonamide and crystallise to constant melting point from dry pet ether (b 90-120°),  then hydrolyse with HCl, excess NaOH is added, and the amine is passed through a column of activated alumina. Redistil the amine and dry it with activated alumina before use [Swift J Am Chem Soc 64115 1942]. [Beilstein 4III 313.]
§ A polystyrene diethylaminomethyl supported version is commercially available.

That is true, but perhaps there is some good hearted chemist out there, that feel it could be right to encourage his/hers fellow clandestine, and upcoming fans of organic chemistry. That is my hope. :D Maybe a bit naive, but never wrong to keep the hopes up :D

Btw Uhrwerk, nice to see that you can supply chems, will most def be in contact with you in the future, for various items :D



Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 07, 2013, 07:20 am
Would there be potential buyers if I sold 1 g for 2000 usd?
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Mcrad on May 07, 2013, 07:36 am
Subscribed:)





Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 07, 2013, 09:14 am
Ok I might put up 10 for sale
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: uhrwerk on May 07, 2013, 11:13 am
the market price in Europe has always been around 200k for a kilo of ergotamine. (its ~50k if you have a license to buy it)
considering the difficulty for acquisition has gone up, i can understand that there is an increase in price.
If you take into account the weight of LSA after hydrolysis of ergotamine is not even 50% and thereafter,  an LSD-synthesis with a yield of 20% can be called a success (according to a knowledgeable chemist at the hive), I dont know if I would be willing to spend 2000EU for a single gram, but the availability is tempting. Customers want to buy grams of L at below 20K you know?
You would need to get a grip of the reaction and the workup, which would waste some further ergotamine to trial and error.
It is an expensive area of synthetic chemistry, but the most interesting one, imho

Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 07, 2013, 01:04 pm
It is very interesting indeed, i was very temped, when i saw the documentary, Albert Hoffmanns  The Substance. When Dr. Hoffmann cut the source from Sandoz to Leary and his friends, Nick Sand educated himself (although having some basic knowledge in Lab and chemistry, he was not a chemist at all) to be a "clandestine" chemist, setting up his first lab in his mothers attic. He made loads of LSD. millions and millions of doses.

Where did they find the culture? Did they just buy it from someone, or did they collect ergot, and grew it themselfs once collected?

2000 EUR per gram seems a bit pricy, i have to say, but still tempting with a source, as mentioned earlier.

I mean, I am interested, not so much of the money that can be made by making LSD, more of the procedure of it. Making it yourself, growing the ergot, extracting and purifying the alkaloids. Exciting stuff indeed. I love chemistry, Dr Hoffmann was a genious!





Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 08, 2013, 04:54 am
Working on it. Def possible but logistics need to be finalised. I know it's pricey but in the right hands it will do much more then 2k. And a product extremely hard to obtain. 
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Limetless on May 08, 2013, 05:00 am
I've had quite a few requests for this and turned them down even though I can get it. Would it really be that popular? I'm hesitant because of working on LSD.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 08, 2013, 05:05 am
I've had quite a few requests for this and turned them down even though I can get it. Would it really be that popular? I'm hesitant because of working on LSD.

LSD is a difficult synthesis. Yes it really does depend on its popularity as I have other purposes for it. Let the posts carry on.....
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Limetless on May 08, 2013, 05:09 am
I've had quite a few requests for this and turned them down even though I can get it. Would it really be that popular? I'm hesitant because of working on LSD.

LSD is a difficult synthesis. Yes it really does depend on its popularity as I have other purposes for it. Let the posts carry on.....

It is indeed but it remains an ongoing struggle for the team. :)

And indeed, I think this thread will either be quite illuminating or fizzle out. We shall have to see...
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 08, 2013, 06:37 am
I think it is very do-able, if there is a great enough interest in the chemist in question, again, look at Nick Sand, the legendary chef of california sunshine  8)

It is just to study, and make the lab equipment you dont have, or cant find (vacuum chamber, magnetic stirring, ect,ect). I assume you guys have seen pictures of Shulgins Lab, It like a small hut, filled with glassware, reminds me of my place, except Shulgin has quite alot more glassware....But im getting there, one step at a time....

As I se it, the greatest issue is the lack of ergot, But there should be some in the fields, summer is coming, just to go out looking on all the fields close by until a source is secured.....Information is available through the web, one can study to almost anything with the information at hand.

After all,  it aint brain surgery ;) Almost, but not quite :D

Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Limetless on May 08, 2013, 06:43 am
I think it is very do-able, if there is a great enough interest in the chemist in question, again, look at Nick Sand, the legendary chef of california sunshine  8)

It is just to study, and make the lab equipment you dont have, or cant find (vacuum chamber, magnetic stirring, ect,ect). I assume you guys have seen pictures of Shulgins Lab, It like a small hut, filled with glassware, reminds me of my place, except Shulgin has quite alot more glassware....But im getting there, one step at a time....

As I se it, the greatest issue is the lack of ergot, But there should be some in the fields, summer is coming, just to go out looking on all the fields close by until a source is secured.....Information is available through the web, one can study to almost anything with the information at hand.

After all,  it aint brain surgery ;) Almost, but not quite :D

Hmmmm might have to consider this carefully.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: randomOVDB#2 on May 08, 2013, 06:47 am
Would there be potential buyers if I sold 1 g for 2000 usd?

No. At that price you are better off buying LSD crystal.

the market price in Europe has always been around 200k for a kilo of ergotamine. (its ~50k if you have a license to buy it)

Pharma prices are around 5-10K for a kilo, definitely not 50k.

I've had quite a few requests for this and turned them down even though I can get it. Would it really be that popular? I'm hesitant because of working on LSD.

Care to share the price you paid for it?

It is very interesting indeed, i was very temped, when i saw the documentary, Albert Hoffmanns  The Substance. When Dr. Hoffmann cut the source from Sandoz to Leary and his friends, Nick Sand educated himself (although having some basic knowledge in Lab and chemistry, he was not a chemist at all) to be a "clandestine" chemist, setting up his first lab in his mothers attic. He made loads of LSD. millions and millions of doses.

Where did they find the culture? Did they just buy it from someone, or did they collect ergot, and grew it themselfs once collected?

They bought ergotamine tartrate from chemical suppliers. It was uncontrolled until the late 80s.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 08, 2013, 06:58 am
if u can front a research & development company in third world or not much restricted/developed country there are few companies in deutsche and swisterland where u can obtain a culture sample legally and the best bet is grow ur own ergots,

at the time hoffman carried it out LSA can be legally bought and the ET too, and if u get a time read operation julie and brotherhood of eternal love which tells how those guys got their hands on the ergot

now the synthesis are getting much easier with new methods such as casey hardisons peptide coupling method, kemps wrinkle cleaning mechanism and many more

i have loads of good reads on the above subject i will be happy to share them and we can definitely sond very source out the chemicals and the precursors  and very soon et, lol
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: Mcrad on May 08, 2013, 07:02 am
if u can front a research & development company in third world or not much restricted/developed country there are few companies in deutsche and swisterland where u can obtain a culture sample legally and the best bet is grow ur own ergots,

at the time hoffman carried it out LSA can be legally bought and the ET too, and if u get a time read operation julie and brotherhood of eternal love which tells how those guys got their hands on the ergot

now the synthesis are getting much easier with new methods such as casey hardisons peptide coupling method, kemps wrinkle cleaning mechanism and many more

i have loads of good reads on the above subject i will be happy to share them and we can definitely sond very source out the chemicals and the precursors  and very soon et, lol

i would love to read these books:)
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: ToadStool on May 08, 2013, 09:32 am
if u can front a research & development company in third world or not much restricted/developed country there are few companies in deutsche and swisterland where u can obtain a culture sample legally and the best bet is grow ur own ergots,

at the time hoffman carried it out LSA can be legally bought and the ET too, and if u get a time read operation julie and brotherhood of eternal love which tells how those guys got their hands on the ergot

now the synthesis are getting much easier with new methods such as casey hardisons peptide coupling method, kemps wrinkle cleaning mechanism and many more

i have loads of good reads on the above subject i will be happy to share them and we can definitely sond very source out the chemicals and the precursors  and very soon et, lol

Actively growing cultures of Claviceps paspali may be purchased from DSMZ Culture Bank in Germany for 100 euros. Here is the link to their online catalog: http://www.dsmz.de/catalogues/details/culture/DSM-885.html?tx_dsmzresources_pi5[returnPid]=304

What we need is someone who works at a legitimate research facility or lab who is willing to purchase a petri dish of live C. paspali, grow additional dishes of the culture under a flowhood and offer them for sale on the road. Anyone interested who has the proper credentials to order please post on this thread. You could make a ton of money just selling cultures. I'm sure someone out here works for a lab or research company and has the authority to order a culture with no trouble at all. It's available, but Joe Blow can't order. Has to be someone with authority working at a legitimate lab capable of submitting the proper paperwork. Any takers?? Let us know - Thanks very much...
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: C20H25N3O on May 08, 2013, 09:56 am
Where we can get a source of claviceps paspali without costing $1000 or more?
Here-there-but-nowhere ..


It will be a pleasure to begin experimenting
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 08, 2013, 11:21 am
Going through the RnD front could be possible but it isn't as easy as it sounds. First of all if your not good at social engineering then forget it. You have to know exactly what your talking about. You see ergot is a highly watched one that company's won't let just go without the correct checks. It's still the best idea so far so worth a try. A rnd company has to be registered with either a vat or eori number.  Iv been through the process and have learnt over the years exactly how to obtain near enough any solvent precursor. This isn't a sales pitch cause I have a different and more effective purpose for the ergotamine.  However people here know who to contact if there is an urgent requirement of ergot or any other solvent intermediates or precursors. Also if anyone needs advise on how to open accounts with company's such sigma Aldrich let me know and I'll guide you.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 08, 2013, 11:24 am
Me no chemist but to whoever doing a research on this field i think this will surely help


A)   Get a source for Claviceps Paspali

B)

L-tryptophane and succinamide have great effect on fermentation.

The best composition of culture medium is 7% sorbitol, 3% succinamide, 0.1% L-tryptophane, 0.05 %MgSO4•7H2O, 0.03% KH2PO4 and pH 5.2 adjusted with ammonia. Through the cultivation of 14 days at 24-26℃ in shake flasks, the biggest content of the ergot alkali reaches 95.6 mg/L

C) Large-scale production

Obtain several ordinary 1 gallon jugs.
Place a two-hole stopper in the necks of the jugs.
Fit a short (6 inch) tube in one hole, leaving two inches above the stopper. Fit a short rubber tube to this. Fill a small (500 ml) Erlenmeyer flask with a dilute solution of sodium hypochlorite (NaClO). Extend a glass tube from the rubber so the end is immersed in the hypochlorite.
Fit a long glass tube in the other stopper hole. It must reach near the bottom of the jug and have about two inches showing above the stopper. Attach a rubber tube to the glass tube and fit a short glass tube to the end of the rubber tube.

Fill a large glass tube (1" x 6") with sterile cotton and fit one-hole stoppers in the ends. Fit the small glass tube in the end of the rubber tube into one stopper of the large tube. Fit another small glass tube into the other stopper. A rubber tube is connected to this and attached to small air pump (obtained from a tropical fish store).
With this aeration equipment you can assure a supply of clean air to the Claviceps Paspali fungus while maintaining a sterile environment inside the solution.
Dismantle the aerators. Place all the glass tubes, rubber tubes, stoppers and cotton in a paper bag, seal tightly with wire staples and sterilize in an autoclave.
Fill the 1-gallon jugs 2/3 to 3/4 full with the culture medium and autoclave.
While these things are being sterilized, homogenize in a blender the culture already obtained and use it to inoculate the material in the gallon jugs. The blender must be sterile.

EVERYTHING must be sterile.

Assemble the aerators. Start the pumps. A slow bubbling in each jug will provide enough oxygen to the cultures. A single pump may be connected to several filters.
Let everything sit at room temperature (25 C) in a dark place (never expose ergot alkaloids to bright light - they will decompose) for a period of ten days.
After ten days, adjust the culture to 1% ethanol using 95% ethanol under sterile conditions. Maintain growth for another two weeks.

D) Extract ergot alkaloids

After a total of 22 days growth period, the culture should be considered mature. Make the culture acidic with tartaric acid and homogenize in a blender for one hour.
Adjust to pH 9 with ammonium hydroxide and extract with benzene or chloroform/iso-butanol mixture.
Extract again with alcoholic tartaric acid and evaporate in a vacuum to dryness.
The dry material is the salt (the tartaric acid salt, the tartrate) of the ergot alkaloids, and is stored in this form because the free basic material is too unstable and decomposes readily in the presence of light, heat, moisture, and air.
To recover the free base for extraction of the amide or synthesis to LSD, make the tartrate basic with ammonia to pH 9, extract with chloroform, and evaporate in vacuo.

Good info for whoever is interested. There's another tek ill post up later also
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 08, 2013, 11:38 am
And as the years past you learn.  We can easily obtain it due to being an official registered company. Even then it doesn't stop. Sometimes there's spot checks and a record of every gram has to be recorded on how it was used. And you would need licensing just for ergotamine. Normally there's 3 category's of licensing. But ergotamine needs a license of its own. From this you are permissible to buy a set amount by the government hence the spot checks. But it is possible. If I can do it anyone can. You just need to know how to do it.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 08, 2013, 01:17 pm

They bought ergotamine tartrate from chemical suppliers. It was uncontrolled until the late 80s.

They might have been bought that to start with, but they had their own cultures, that i am sure of....

Shulginsindex: Is that the normal procedure all over the world? I have a hard time believing that, still maaaany places you can buy it all with just some good old paper money. Offcourse some substances are very watched, but when they originate from mother nature, there is always a way to get the starting material, if the species isnt extinct....
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: blueveil on May 08, 2013, 04:19 pm
If we could receive one safely, we would gladly grow it out and distribute ergot for close to free (minus actual costs like shipping and agar medium)....... We have the facilities and equipment to do this safely, but cannot come up with a safe way to receive the sample. Been cultivating different microbes for decades, but would love to dabble with some REAL DEAL ergot.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: WeSaySo on May 08, 2013, 08:06 pm
you chatty cathys never really take the time to think ahead do you?

Do you know how sources get fucked off? by talking about them.

Do you know the exact reason why the DEA was created? The Brotherhood....

If theres any sources in this thread that are real, by posting about them here, you have ensured they will be more than trip-wired now. good job. good luck.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 08, 2013, 10:15 pm
you chatty cathys never really take the time to think ahead do you?

Do you know how sources get fucked off? by talking about them.

Do you know the exact reason why the DEA was created? The Brotherhood....

If theres any sources in this thread that are real, by posting about them here, you have ensured they will be more than trip-wired now. good job. good luck.

we believe its the freedom of humanity to get educate ourselves on the interesting fields even though they are illegal and again even with the dea around still we get all the drugs we need,lol and its just usa and there are many places in the planet the dea doesnt have a clue about what the shit is cooking

and sharing ideas makes the process simpler and obviously dea needs to recruit/spend more people/time/resources to check on the new avenues, its fun isnt it to keep the pigs busy??


anyways the ergot still can be freely find in many rye,wheat and bhai grass fields growing without no ones intervention and dr.kim yes still u can get ur hands on ET with paper money if u have proper contacts, heard most of blackmarket et  comes out from czech republic

Title: Re: ERGOT CULTURE, or ergot derivative
Post by: WeSaySo on May 09, 2013, 12:14 am
if you think the dea cant pressure people in other countries than your fucklng pretty dumb.

your not gonna waste their time. they will just make them stop offering it.

people like you fuck everything up for people that are actually doing things and not chatting about them on SR forums like a bunch of fucking teenage girls. If you want to learn about LSD then pick up a fucking book.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: echo_ on May 09, 2013, 01:01 am
I would like to offer my love and appreciation for those souls interested in the cultivation of ergot. It is a heroic deed. I wish you safety.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: jameslink2 on May 09, 2013, 01:45 am
Actively growing cultures of Claviceps paspali may be purchased from DSMZ Culture Bank in Germany for 100 euros. Here is the link to their online catalog: http://www.dsmz.de/catalogues/details/culture/DSM-885.html?tx_dsmzresources_pi5[returnPid]=304

What we need is someone who works at a legitimate research facility or lab who is willing to purchase a petri dish of live C. paspali, grow additional dishes of the culture under a flowhood and offer them for sale on the road. Anyone interested who has the proper credentials to order please post on this thread. You could make a ton of money just selling cultures. I'm sure someone out here works for a lab or research company and has the authority to order a culture with no trouble at all. It's available, but Joe Blow can't order. Has to be someone with authority working at a legitimate lab capable of submitting the proper paperwork. Any takers?? Let us know - Thanks very much...

I dont have the balls to order it to my lab but I have the degree, ability, lab, flowhoods, incubators, etc to grow it out. I just dont have a sample of it.

Though if there is a call I may take a trip and search to see what I find.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 09, 2013, 05:25 am
This will  be magnificent! I think the ancient molecules will find their way to us, already 2 willing mycologists are popping up, like mushrooms in the field :D The ergot will come along!

Imagine people doing this in the late 60s! They might have had easier access to chems, but no internet then. We have all the information needed, just a click away.

Information wants to be free!

Erowid is amazingly full of nutrition for a hungry mind, http://www.erowid.org/archive/rhodium/chemistry/lsd.synthesis.html







Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 09, 2013, 05:49 am
if you think the dea cant pressure people in other countries than your fucklng pretty dumb.

your not gonna waste their time. they will just make them stop offering it.

people like you fuck everything up for people that are actually doing things and not chatting about them on SR forums like a bunch of fucking teenage girls. If you want to learn about LSD then pick up a fucking book.

DEA can take a good look at ur butt while we figure this out,lol, Silkroad is all about freedom and not for couple of faggots  who thinks they can run the show behind a curtain, and its pathetic that people from states thinks dea is everything, fuckall mate grow up ,

people like us make it easier and clear for the people who really has balls to do it, lets call it evolution of drugs and we believe collectively we can do the humanity a favour by spreading more lsd u are a pussy no doubt or le himself, go fuck urself mate
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: DrDeepWood on May 09, 2013, 05:56 am
I offer a source for a very nice strain of ergot when you buy my LSD lab guide.  The source is on SR and works inside of escrow, much better than getting your door kicked in trying to order from ATCC.

Don't even think for a second you can use wild ergot to produce ergotamine, strains are selected over many generations to get a producer.  My strain is the producer and will make around 1.5 grams for every liter of fermentation broth.  This is impossible with wild strains.

Also my strain is claviceps paspali which is superior to claviceps purpurea due to the fact that paspali produces more water soluble erogolines which are much less poisonous and more easy flow from the cell, allowing higher yields.

I will sell the source without the guide for 250$, my source charges around 300$ for a plate shipped.

If you are interested in fermentation I would suggest you buy my guide, it will pay for itself due to the fact that it includes information on ion exchange resins.  You can extract from a fermentation solution using only water and a little alcohol!
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 09, 2013, 07:22 am
I offer a source for a very nice strain of ergot when you buy my LSD lab guide.  The source is on SR and works inside of escrow, much better than getting your door kicked in trying to order from ATCC.

Don't even think for a second you can use wild ergot to produce ergotamine, strains are selected over many generations to get a producer.  My strain is the producer and will make around 1.5 grams for every liter of fermentation broth.  This is impossible with wild strains.

Also my strain is claviceps paspali which is superior to claviceps purpurea due to the fact that paspali produces more water soluble erogolines which are much less poisonous and more easy flow from the cell, allowing higher yields.

I will sell the source without the guide for 250$, my source charges around 300$ for a plate shipped.

If you are interested in fermentation I would suggest you buy my guide, it will pay for itself due to the fact that it includes information on ion exchange resins.  You can extract from a fermentation solution using only water and a little alcohol!

Hello Mr Deepwood! nice of you to join in our discussion!

Some few questions, if you dont mind.

What is the time limit for starting a fermenting broth with the CP you supply?

How far ahead would you recommend studying your manual, and set up all necessary things prior to starting a fermentation?

What would the most important parts of the lab equipment consist of, according to you?

How is your manual set up? Does it cover all steps, from ergot cultivation, to extraction of alkaloids, and synthesis of LSD from those aklaloids, aswell as purification of the lsd once synthesised? 

I have been a bit suprised from the lack of reviews from your manual/ergot source. Maybe people just keeping it stealthy, who knows? But still, would be nice to see some verification of a successful synthesis. I might just order it from you, and be that source.



Title: Re: ERGOT CULTURE, or ergot derivative
Post by: shulginsindex on May 09, 2013, 08:06 am
if you think the dea cant pressure people in other countries than your fucklng pretty dumb.

your not gonna waste their time. they will just make them stop offering it.

people like you fuck everything up for people that are actually doing things and not chatting about them on SR forums like a bunch of fucking teenage girls. If you want to learn about LSD then pick up a fucking book.

DEA can take a good look at ur butt while we figure this out,lol, Silkroad is all about freedom and not for couple of faggots  who thinks they can run the show behind a curtain, and its pathetic that people from states thinks dea is everything, fuckall mate grow up ,

people like us make it easier and clear for the people who really has balls to do it, lets call it evolution of drugs and we believe collectively we can do the humanity a favour by spreading more lsd u are a pussy no doubt or le himself, go fuck urself mate

Agreed. No one is specifically mentioning sources.  It's vague but enough for an experienced member to understand. Chemical purchasing and infrastructure information is very important. Also laws.

Oh and dr deepwood no offence but your tek isn't viable for the average chemist. If you added Information on sources to obtain chemicals needed for the synthesis it would half your tek and make life easier for your customer. But the tek is well detailed and had some interesting points.  But I just can't imagine the average chemist getting it done. 
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: randomOVDB#2 on May 09, 2013, 12:24 pm
if you think the dea cant pressure people in other countries than your fucklng pretty dumb.

your not gonna waste their time. they will just make them stop offering it.

people like you fuck everything up for people that are actually doing things and not chatting about them on SR forums like a bunch of fucking teenage girls. If you want to learn about LSD then pick up a fucking book.

DEA can take a good look at ur butt while we figure this out,lol, Silkroad is all about freedom and not for couple of faggots  who thinks they can run the show behind a curtain, and its pathetic that people from states thinks dea is everything, fuckall mate grow up ,

people like us make it easier and clear for the people who really has balls to do it, lets call it evolution of drugs and we believe collectively we can do the humanity a favour by spreading more lsd u are a pussy no doubt or le himself, go fuck urself mate

Agreed. No one is specifically mentioning sources.  It's vague but enough for an experienced member to understand. Chemical purchasing and infrastructure information is very important. Also laws.

He is talking about the DSMZ link in post #30.

shulginsindex isn't that tek more or less a rewrite of Hardison's writeup? From lysergic acid onwards.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: ToadStool on May 09, 2013, 05:02 pm
you chatty cathys never really take the time to think ahead do you?

Do you know how sources get fucked off? by talking about them.

Do you know the exact reason why the DEA was created? The Brotherhood....

If theres any sources in this thread that are real, by posting about them here, you have ensured they will be more than trip-wired now. good job. good luck.

Why so negative WeSaySo? We aren't asking anyone to reveal clandestine sources. We are asking for someone to make culture samples available for sale on the road if they have it. As for the link to the culture bank, it comes up with a simple Startpage search for Claviceps paspali. The culture bank maintains live slants for sale but they don't or won't sell certain restricted organisms to anyone except professionals with the necessary credentials. Everyone knows what Claviceps is used for... we're not revealing any secrets here.
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 09, 2013, 05:57 pm
you chatty cathys never really take the time to think ahead do you?

Do you know how sources get fucked off? by talking about them.

Do you know the exact reason why the DEA was created? The Brotherhood....

If theres any sources in this thread that are real, by posting about them here, you have ensured they will be more than trip-wired now. good job. good luck.


Why so negative WeSaySo? We aren't asking anyone to reveal clandestine sources. We are asking for someone to make culture samples available for sale on the road if they have it. As for the link to the culture bank, it comes up with a simple Startpage search for Claviceps paspali. The culture bank maintains live slants for sale but they don't or won't sell certain restricted organisms to anyone except professionals with the necessary credentials. Everyone knows what Claviceps is used for... we're not revealing any secrets here.



Good try you guys, but I dont think WeSaySo will listen to much, he seems an upset guy. He has asked me to close the thread down 3 days ago, I sincerely explained what everyone else here has written, but he still insist that i close it down, and I will get the blame for stealth vendors disappearing as a direct cause of my thread topic.







 
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: indica9 on May 10, 2013, 05:37 am
A high yielding (1.5g/L) Claviceps Paspali culture on a PDA plate is now available on SR
http://silkroadvb5piz3r.onion/silkroad/item/4b9976968a

Is this the same source the dr.deepwood was telling about?? but he said its around usd300 but this is usd800
do u like to share some info on the cultivation of it mate??
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: randomOVDB#2 on May 10, 2013, 08:16 am
A high yielding (1.5g/L) Claviceps Paspali culture on a PDA plate is now available on SR
http://silkroadvb5piz3r.onion/silkroad/item/4b9976968a

ATCC classification number?
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: echo_ on May 10, 2013, 01:25 pm
I just looked it up and couldn't find it, it is possible the thread has been removed. Luckily, I saved the page.
https://www.thevespiary.org/talk/index.php/topic,1294.0.html
Title: Re: ERGOT CULTURE, or ergot derivative
Post by: drkim on May 11, 2013, 08:29 pm
almost OT, but still, very good video series of BASIC organic chemistry, with a very nice understandable teacher, is found at

http://video.answers.com/learn-about-organic-chemistry-1-111430091

I liked it a lot, a good thing to watch, if you are interested in organic chemistry, and doesnt have a heavy university education behind you.

Enjoy